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Original Article
Study of targeting fibrin polypeptide nanoprobes in enhanced MRI for venous thrombosis
WU Hongyun  SHI Yonggui  LIAO Yukun  HE Honglin  ZHONG Yixin 

Cite this article as: Wu HY, Shi YG, Liao YK, et al. Study of targeting fibrin polypeptide nanoprobes in enhanced MRI for venous thrombosis[J]. Chin J Magn Reson Imaging, 2022, 13(12): 117-123. DOI:10.12015/issn.1674-8034.2022.12.020.

[Abstract] Objective The individualized treatment of patients with venous thromboembolism is closely related to their good prognosis, and accurate diagnosis is necessary for the correct formulation of treatment plans. Given the high biological safety of ferric oxide and polylactic hydroxyacetic acid (PLGA), we designed a nanoprobe (NP) of ferric oxide polylactic hydroxyacetic acid pentapeptide (Fe3O4-PLGA-CREKA) to improve the detection ability of thrombus through targeting fibrin.Materials and Methods Fe3O4-PLGA-CREKA nanoprobes were made by double emulsification and carbodiimide, and their physicochemical properties were tested. In-vitro magnetic resonance imaging (MRI) of Fe3O4-PLGA-CREKA nanoprobe was performed with T2WI and T2 mapping sequences to analyze the correlation between nanoprobe concentration and T2 relaxation rate. The venous blood of rats was collected to make frozen sections and incubated with Fe3O4-PLGA/DiI CREKA NPs (targeted group) and Fe3O4-PLGA/DiI NPs (non-targeted group) respectively. The targeting of different nanoprobes to thrombus was observed by inverted fluorescent microscope. After the safety verification of the nanoprobe on cells, a venous thrombosis model was established in the jugular vein of SD rats. Fe3O4-PLGA-CREKA NP (targeted group) and Fe3O4-PLGA NP (non-targeted group) were injected into the caudal vein respectively. Data were collected after T2WI and T2 mapping sequence scanning. At the same time, major organs were collected, and the safety of the nanoprobe was verified by H&E staining.Results In this study, Fe3O4-PLGA-CREKA nanoprobes with a particle size of (255.3±56.0) nm were successfully prepared, and their surface potential was (-18.90±5.84) mV; The TEM results showed that the nanoprobe was a homogeneous sphere with good dispersion; The nanoprobes had the ability to enhance MR signal; Compared with the non-targeted group, the targeted group's nanoprobes can better target venous thrombosis, which has good biosafety; the T2 relaxation rates of non-targeted group and the targeted group before and after administration were (17.33±2.25) s-1, (49.00±6.66) s-1, (19.00±3.90) s-1 and (72.83±6.68) s-1, respectively.Conclusions The Fe3O4-PLGA-CREKA nanoprobes prepared in our study possess good biosafety and MRI imaging ability with high specificity for venous thrombosis, which is expected to help with venous thromboembolism diagnosis and treatment in the future.
[Keywords] nanoprobe;thrombus targeting;magnetic resonance imaging;pentapeptide;multimodal imaging

WU Hongyun   SHI Yonggui   LIAO Yukun   HE Honglin   ZHONG Yixin*  

Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

Zhong YX, E-mail:

Conflicts of interest   None.

ACKNOWLEDGMENTS Postdoctoral Natural Science Foundation of Chongqing (No. cstc2021jcyj-bshX0136); China Postdoctoral Science Foundation (No. 2019M663891XB); The Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University (No. kryc-gg-2213).
Received  2022-07-06
Accepted  2022-11-29
DOI: 10.12015/issn.1674-8034.2022.12.020
Cite this article as: Wu HY, Shi YG, Liao YK, et al. Study of targeting fibrin polypeptide nanoprobes in enhanced MRI for venous thrombosis[J]. Chin J Magn Reson Imaging, 2022, 13(12): 117-123. DOI:10.12015/issn.1674-8034.2022.12.020.

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