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Original Articles
Quantitative analysis of early changes in the myelin structure of rats with ischemic stroke using MRI signal intensity ratio and diffusion tensor imaging
WENG Na  SHAO Cuijie  LI Guodong  BIN Li  HUANG Danqi  WANG Xu 

Cite this article as: Weng N, Shao CJ, Li GD, et al. Quantitative analysis of early changes in the myelin structure of rats with ischemic stroke using MRI signal intensity ratio and diffusion tensor imaging[J]. Chin J Magn Reson Imaging, 2022, 13(10): 150-156. DOI:10.12015/issn.1674-8034.2022.10.023.


[Abstract] Objective To explore the early diagnostic value of signal intensity ratio (SIR) and diffusion tensor imaging (DTI) in myelin structural changes in the white matter of ischemic stroke rats.Materials and Methods Eighteen Sprague Dawley rats were divided into three groups, Groups A, B, and C, with six rats in each group. Middle cerebral artery occlusion (MCAO) sham operation without ligation was performed in group A, the 24 hours after MCAO operation in group B, and the 72 hours after MCAO operation in group C. After obtaining T1WI, T2WI, and DTI scans under the same conditions, post-processing to get the SIR, fractional anisotropy (FA), apparent diffusion coefficient (ADC), radial diffusivity (D⊥) imaging. The corpus callosum and external capsule regions were outlined to calculate the SIR value, FA value, ADC value, and D⊥ value, and a one-way analysis of variance was performed among the three groups. At the end of scanning, the samples were obtained for luxol fast blue (LFB) -tar purple pathological staining, and the mean grayscale value (MGSV) was measured. One-way analysis of variance between the three groups was performed. Pairwise correlation analysis was performed between the statistically significant imaging data and the MGSV.Results Compared with group A, group B had decreased SIR (P=0.044), fractional anisotropy (FA; P=0.001), apparent diffusion coefficient (ADC; P=0.001) and radial diffusion coefficient (D⊥; P=0.001) values in the external capsule region. Group C had decreased SIR (P<0.001) and FA (P=0.002) in the external capsule region. SIR (P=0.031) and FA (P=0.015) in the corpus callosum were decreased. LFB-tar purple poorly stained the areas of the myelin sheath that were damaged due to stroke, and the mean grayscale value (MGSV) decreased (P<0.05). In the external capsule region, MGSV was negatively correlated with SIR (P<0.0001), FA (P≤0.0001), ADC (P=0.0127), and D⊥ (P=0.0180). In the corpus callosum region, MGSV was negatively correlated with SIR (P=0.0018).Conclusions A combination of SIR and DTI can be used as a noninvasive imaging method to evaluate the different stages of myelin injury in ischemic stroke. This has been proven effective in rat models and provides a helpful imaging basis for clinical diagnosis and treatment.
[Keywords] ischemia stroke;demyelination;MCAO model;signal intensity ratio;magnetic resonance imaging;diffusion tensor imaging

WENG Na1   SHAO Cuijie2   LI Guodong1   BIN Li1   HUANG Danqi1   WANG Xu1*  

1 Department of Nuclear Medicine, Binzhou Medical University Hospital, Binzhou 256600, China

2 Medical Research Center, Binzhou Medical University Hospital, Binzhou 256600, China

Wang X, E-mail:wangxu1978@163.com

Conflicts of interest   None.

Received  2022-07-05
Accepted  2022-09-30
DOI: 10.12015/issn.1674-8034.2022.10.023
Cite this article as: Weng N, Shao CJ, Li GD, et al. Quantitative analysis of early changes in the myelin structure of rats with ischemic stroke using MRI signal intensity ratio and diffusion tensor imaging[J]. Chin J Magn Reson Imaging, 2022, 13(10): 150-156.DOI:10.12015/issn.1674-8034.2022.10.023

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