Share this content in WeChat
Original Article
Compared study on the efficacy and safety of MR and CT guided percutaneous VX2 hepatic para-vascular tumor model in rabbits
FENG Xu  FENG Guiling  LIU Yan  SUN Baijintao  LI Bing  DU Yong 

Cite this article as: Feng X, Feng GL, Liu Y, et al. Compared study on the efficacy and safety of MR and CT guided percutaneous VX2 hepatic para-vascular tumor model in rabbits[J]. Chin J Magn Reson Imaging, 2022, 13(4): 84-88. DOI:10.12015/issn.1674-8034.2022.04.015.

[Abstract] Objective To compare the efficacy and safety of 1.5 T closed magnetic resonance (MR) and computed tomography (CT) guided VX2 hepatic para-vascular tumor model in rabbits.Materials and Methods Sixty New Zealand white rabbits were randomly and equally divided into MR-guided group (n=30) and CT-guided group (n=30). Rabbit VX2 tumor fragments was implanted beside the rabbit hepatic great vessels under MR and CT-guidance in the MR and CT group respective, to evaluate the success rate of tumor model establishment, puncture needle pseudopacity/artifact and vascular development around the needle tip, operation time and safety.Results The success rate of MR-guided modeling was 96.7%, only 1 rabbit had needle metastasis, the success rate of CT-guided modeling was 80.0%, 2 rabbits had needle metastasis, 3 rabbits had metastases in other parts of the liver, and 1 had unknown cause of death. The differences in success rate of tumor model establishment between the two groups was statistically significant (P=0.044). The fold number of artifacts at T1 weighted imaging (T1WI) was 7.26±0.38 for the 20 G coaxial puncture needle in the MR-guided group and 2.51±0.57 for the 20 G coaxial puncture needle in the CT-guided group, and the difference was statistically significant (P<0.01), but 13 rabbits (43.3%) in the CT-guided group showed star-shaped hypodense artifacts would appear around the needle tip, which interfered with intrahepatic vascular display, and the difference was statistically significant (χ2=8.523, P<0.01). The operation time was longer in the MR-guided group than in the CT-guided group (13.32±2.45) min in the MR-guided group vs. (8.42±1.46) min in the CT-guided group; P<0.001). A small amount of ascites occurred in 2 patients (6.67%) in the CT-guided group. No serious complications such as liver abscess, jaundice or diaphragmatic perforation were observed in either groups.Conclusions Compared with CT, MR-guided hepatic para-vascular tumor implantation in rabbits might be a more effective modeling method. Although the needle tip pseudopacity of puncture needle is large and the operation takes a long time, the vascular development around the needle tip is clear and the incidence of complications is low.
[Keywords] magnetic resonance imaging;computed tomography;hepatic para-vascular tumor model;rabbit VX2 tumor;success rate of tumor model establishment;safety

FENG Xu   FENG Guiling   LIU Yan   SUN Baijintao   LI Bing   DU Yong*  

Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China

Du Y, E-mail:

Conflicts of interest   None.

Received  2021-08-16
Accepted  2022-03-21
DOI: 10.12015/issn.1674-8034.2022.04.015
Cite this article as: Feng X, Feng GL, Liu Y, et al. Compared study on the efficacy and safety of MR and CT guided percutaneous VX2 hepatic para-vascular tumor model in rabbits[J]. Chin J Magn Reson Imaging, 2022, 13(4): 84-88.DOI:10.12015/issn.1674-8034.2022.04.015

Fleming BD, Urban DJ, Hall MD, et al. Engineered anti-GPC3 immunotoxin, HN3-ABD-T20, produces regression in mouse liver cancer xenografts through prolonged serum retention[J]. Hepatology, 2020, 71(5): 1696-1711. DOI: 10.1002/hep.30949.
Craig AJ, Johann VF, Teresa GL, et al. Tumour evolution in hepatocellular carcinoma[J]. Nat Rev Gastroenterol Hepatol, 2020, 17(3): 139-152. DOI: 10.1038/s41575-019-0229-4.
Biondetti P, Fumarola EM, Ierardi AM, et al. Percutaneous US-guided MWA of small liver HCC: predictors of outcome and risk factors for complications from a single center experience[J]. Med Oncol, 2020, 37(5): 39. DOI: 10.1007/s12032-020-01365-x.
Suwa K, Seki T, Aoi K, et al. Efficacy of microwave ablation versus radiofrequency ablation for hepatocellular carcinoma: a propensity score analysis[J]. Abdom Radiol (NY), 2021, 46(8): 3790-3797. DOI: 10.1007/s00261-021-03008-9.
Nault JC, Sutter O, Nahon P, et al. Percutaneous treatment of hepatocellular carcinoma: state of the art and innovations[J]. J Hepatol, 2018, 68(4): 783-797. DOI: 10.1016/j.jhep.2017.10.004.
Cao SJ, Lyu TS, Fan ZY, et al. Long-term outcome of percutaneous radiofrequency ablation for periportal hepatocellular carcinoma: tumor recurrence or progression, survival and clinical significance[J]. Cancer Imaging, 2022, 22(1): 2. DOI: 10.1186/s40644-021-00442-2.
Izzo F, Granata V, Grassi R, et al. Radiofrequency ablation and microwave ablation in liver tumors: an update[J]. Oncologist, 2019, 24(10): e990-e1005. DOI: 10.1634/theoncologist.2018-0337.
Qiao W, Yu YL, Huang Y, et al. Impact of focused ultrasound on the ethanol ablation of VX2 liver tumours in rabbits[J]. Eur Radiol, 2020, 30(11): 5862-5870. DOI: 10.1007/s00330-020-06941-3.
Khabbaz RC, Huang YH, Smith AA, et al. Development and angiographic use of the rabbit VX2 model for liver cancer[J]. J Vis Exp, 2019(143). DOI: 10.3791/58600.
Wan RJ, Lin ZY, Li YM, et al. Establishing the rabbit model bearing VX2 tumor near blood vessel in liver under the guidance of MRI[J]. J Med Imaging, 2014, 24(5):865-867.
van Breugel JMM, Geschwind JF, Mirpour S, et al. Theranostic application of lipiodol for transarterial chemoembolization in a VX2 rabbit liver tumor model[J]. Theranostics, 2019, 9(13): 3674-3686. DOI: 10.7150/thno.32943.
Lu CY, (Xi/Hui) JG, Xia SW, et al. Application values of 64-row computed tomographic angiography in diagnosing liver VX2 tumor in rabbits[J]. Natl Med J China, 2015, 95(7): 541-543. DOI: 10.3760/cma.j.issn.0376-2491.2015.07.016.
Wang GR, Gao QZ, Wang ZW, et al. Reduction of microwave ablation needle related metallic artifacts using virtual monoenergetic images from dual-layer detector spectral CT in a rabbit model with VX2 tumor[J]. Sci Rep, 2021, 11(1): 9295. DOI: 10.1038/s41598-021-88853-w.
Chen TM, Pan YZ. Establishing the rabbit liver VX2 tumor model using CT-guided percutaneously transhepatic puncture inoculation and evaluating the model using MRI and CT[J]. J Guizhou Med Univ, 2021, 46(6): 699-706. DOI: 10.19367/j.cnki.2096-8388.2021.06.013.
Meloni MF, Galimberti S, Dietrich CF, et al. Microwave ablation of hepatic tumors with a third generation system: loco-regional efficacy in a prospective cohort study with intermediate term follow-up[J]. Z Gastroenterol, 2016, 54(6): 541-547. DOI: 10.1055/s-0042-100627.
Song HS, Ding HY, Zhu CD. CT-guided percutaneous microwave ablation of sclerosing hepatic carcinoma[J]. Can J Gastroenterol Hepatol, 2020, 2020: 8881978. DOI: 10.1155/2020/8881978.
Hoffmann R, Rempp H, Eibofner F, et al. In vitro artefact assessment of a new MR-compatible microwave antenna and a standard MR-compatible radiofrequency ablation electrode for tumour ablation[J]. Eur Radiol, 2016, 26(3): 771-779. DOI: 10.1007/s00330-015-3891-0.
van Dams R, Wu TC, Kishan AU, et al. Ablative radiotherapy for liver tumors using stereotactic MRI-guidance: a prospective phase I trial[J]. Radiother Oncol, 2021: S0167-S8140(21)06577-4. DOI: 10.1016/j.radonc.2021.06.005.
Xu KH, Wang YP, Wang CY, et al. Closed MRI-guided microwave ablation of subdiaphragmatic malignant liver malignanttumors under general laryngeal mask anesthesia[J]. Chin J Interv Imaging Ther, 2021, 18(3): 129-132. DOI: 10.13929/j.issn.1672-8475.2021.03.001.
Boldrini L, Corradini S, Gani, et al. MR-guided radiotherapy for liver malignancies[J]. Front Oncol, 2021, 11: 616027. DOI: 10.3389/fonc.2021.616027.
Li ZN, Wang CY, Si GY, et al. Image-guided microwave ablation of hepatocellular carcinoma (≤5.0 cm): is MR guidance more effective than CT guidance?[J]. BMC Cancer, 2021, 21(1): 366. DOI: 10.1186/s12885-021-08099-7.
Shin SW, Ahn KS, Kim SW, et al. Liver resection versus local ablation therapies for hepatocellular carcinoma within the Milan criteria: a systematic review and meta-analysis[J]. Ann Surg, 2021, 273(4): 656-666. DOI: 10.1097/SLA.0000000000004350.
Cha DI, Lee MW, Kang TW, et al. Comparison between CT and MR images as more favorable reference data sets for fusion imaging-guided radiofrequency ablation or biopsy of hepatic lesions: a prospective study with focus on patient's respiration[J]. Cardiovasc Intervent Radiol, 2017, 40(10): 1567-1575. DOI: 10.1007/s00270-017-1666-5.

PREV Study on the change of resting state degree centrality and correlation in patients with obstructive sleep apnea
NEXT A comparative study on phantom verification of T1 and T2 relaxation values determined by synthetic MRI and conventional mapping methods

Tel & Fax: +8610-67113815    E-mail: