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Original Article
Application of magnetic nanoparticles in vitro MRI of mouse macrophages
SONG Jiang  GE Rui  ZHU Kai  SUN Jie  SONG Meina  ZHAO Wei  MA Hongning  WANG Zhijun 

Cite this article as: Song J, Ge R, Zhu K, et al. Application of magnetic nanoparticles in vitro MRI of mouse macrophages[J]. Chin J Magn Reson Imaging, 2022, 13(2): 57-61. DOI:10.12015/issn.1674-8034.2022.02.012.


[Abstract] Objective To investigate the optimal concentration of mouse macrophage cell line RAW264.7 labeled with ultra-micro superparamagnetic iron oxide nanoparticles (USPIO) and to compare the sensitivity differences of different scanning sequences in the evaluation of cell phagocytosis in MRI imaging.Materials and Methods The final concentrations of USPIO (0, 25, 50, 75, 100, 125 μg/mL) were co-cultured with mouse macrophages for 24 h and then the cell viability was calculated using the cell counting reagent (CCK-8) as well as the half inhibitory concentration (IC50) of USPIO on the cells. The morphological changes of the cells were observed under a light microscope. Prussian blue staining was used to confirm the phagocytic effect of cells on USPIO. 3.0 T MRI scan of the cell-agarose gel model was performed to record the relaxation time and relaxation rate of T1WI and T2WI sequences and calculate the reduction rate of relaxation time.Results When the concentration of USPIO was 25 μg/mL, there was no effect on the cell viability and the difference was not statistically significant (P>0.05). When the concentration of USPIO was ≥50 μg/mL, the cell viability was significantly decreased with the increase of USPIO concentration (all P<0.05). The IC50 for the median inhibitory concentration of USPIO on cells was (186.5±7.2) μg/mL. When the concentration of USPIO was 50 μg/mL, the cell morphology began to shrink and the light transmittance decreased. When the concentration of USPIO was 25 μg/mL, the Prussian blue staining was significantly positive. MRI imaging showed significant signal changes in the cells at a concentration of USPIO of 25 μg/mL compared to the control group; With the increase of USPIO concentration, the relaxation times of T1 and T2 were significantly shortened (all P<0.01), and the corresponding relaxation rates R1 and R2 were gradually increased. Under the same concentration of USPIO, the reduction rates of T2 relaxation time in each group were significantly higher than that of T1 relaxation time (all P<0.001).Conclusions USPIO at a concentration of 25 μg/mL has no obvious toxic effect on cells, and it is efficient in labeling, with obvious signal changes on MRI and good imaging effect. It is the optimal concentration for labeling macrophages. MRI can be used for imaging in vitro after cell labeling, and the signal changes of T2WI sequence after detecting the phagocytosis of USPIO by cells are superior to that of T1WI sequence.
[Keywords] macrophages;ultra-micro superparamagnetic iron oxide nanoparticles;magnetic resonance imaging;mouse

SONG Jiang1, 2   GE Rui1   ZHU Kai1   SUN Jie1   SONG Meina1   ZHAO Wei2, 3   MA Hongning2   WANG Zhijun1*  

1 Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan 750004, China

2 Central Laboratory of People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750004, China

3 Basic Medical College of Ningxia Medical University, Yinchuan 750004, China

Wang ZJ, E-mail: wangzhijun2056@163.com

Conflicts of interest   None.

Received  2021-09-09
Accepted  2021-12-28
DOI: 10.12015/issn.1674-8034.2022.02.012
Cite this article as: Song J, Ge R, Zhu K, et al. Application of magnetic nanoparticles in vitro MRI of mouse macrophages[J]. Chin J Magn Reson Imaging, 2022, 13(2): 57-61.DOI:10.12015/issn.1674-8034.2022.02.012

[1]
Zhang JS, Zhang Y, Xin SF, et al. CXCR7 suppression modulates macrophage phenotype and function to ameliorate post-myocardial infarction injury[J]. Inflamm Res, 2020, 69(5): 523-532. DOI: 10.1007/s00011-020-01335-z.
[2]
Yang YN, Ren GH, Wang ZF, et al. Human cytomegalovirus IE2 protein regulates macrophage-mediated immune escape by upregulating GRB2 expression in UL122 genetically modified mice[J]. Biosci Trends, 2020, 13(6): 502-509. DOI: 10.5582/bst.2019.01197.
[3]
Fang WL, Zhou T, Shi H, et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 T cell exclusion[J] .J Exp Clin Cancer Res, 2021, 40(1): 4. DOI: 10.1186/s13046-020-01786-6.
[4]
Benzin H, Schumann S, Richter A, et al. Evaluation of Human Skin-Derived Stem Cell Characteristics After Non-Invasive Quantum Dot Labeling[J]. Cell Physiol Biochem, 2021, 55(4): 387-399. DOI: 10.33594/000000391.
[5]
Sato N, Stringaris K, Davidson-Moncada JK, et al. In Vivo Tracking of Adoptively Transferred Natural Killer Cells in Rhesus Macaques Using Zirconium-Oxine Cell Labeling and PET Imaging[J] .Clin Cancer Res, 2020, 26(11): 2573-2581. DOI: 10.1158/1078-0432.CCR-19-2897.
[6]
Chen C, Ge J, Gao Y, et al. Ultrasmall superparamagnetic iron oxide nanoparticles: A next generation contrast agent for magnetic resonance imaging[J]. 2021(undefined): e1740. DOI: 10.1002/wnan.1740.
[7]
Merinopoulos I, Gunawardena T, Stirrat C, et al. Diagnostic Applications of Ultrasmall Superparamagnetic Particles of Iron Oxide for Imaging Myocardial and Vascular Inflammation[J]. JACC Cardiovasc Imaging, 2021, 14(6): 1249-1264. DOI: 10.1016/j.jcmg.2020.06.038.
[8]
Wang YS, Liu HH, Yao DF, et al. F-labeled magnetic nanoparticles for monitoring anti-angiogenic therapeutic effects in breast cancer xenografts[J]. J Nanobiotechnology, 2019, 17(1): 105. DOI: 10.1186/s12951-019-0534-7.
[9]
Hwang YH, Kim YJ, Lee DY. Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles[J]. Biomater Res, 2021, 25(1): 36. DOI: 10.1186/s40824-021-00241-7.
[10]
Ohki A, Saito S, Fukuchi K. Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters[J]. Sci Rep, 2020, 10(1): 13684. DOI: 10.1038/s41598-020-70291-9.
[11]
Belkahla H, Antunes JC, Lalatonne Y, et al. USPIO-PEG nanoparticles functionalized with a highly specific collagen-binding peptide: a step towards MRI diagnosis of fibrosis[J]. J Mater Chem B, 2020, 8(25): 5515-5528. DOI: 10.1039/d0tb00887g.
[12]
Staitieh BS, Auld SC, Ahmed M, et al. Granulocyte Macrophage-Colony Stimulating Factor Reverses HIV Protein-Induced Mitochondrial Derangements in Alveolar Macrophages[J]. 2021, 37(3): 224-232. DOI: 10.1089/aid.2020.0176.
[13]
Gensollen T, Lin X, Zhang T, et al. Embryonic macrophages function during early life to determine invariant natural killer T cell levels at barrier surfaces[J]. 2021, 22(6): 699-710. DOI: 10.1038/s41590-021-00934-0.
[14]
Fultz R, Engevik MA, Shi Z, et al. Phagocytosis by macrophages depends on histamine H2 receptor signaling and scavenger receptor 1[J]. 2019, 8(10): e908. DOI: 10.1002/mbo3.908.
[15]
Helmer P, Damm E, Schiekofer S, et al. β3-integrin Leu33Pro gain of function variant does not modulate inflammatory activity in human derived macrophages in diabetes[J]. Int J Med Sci, 2021, 18(12): 2661-2665. DOI: 10.7150/ijms.55648.
[16]
Wang F, Xiong Y, He H, et al. Screening of Optimal Concentration of Magnetic Labeled Placental Mesenchymal Stem Cells[J]. Journal of Ningxia Medical University, 2020, 42(2): 128-134. DOI: 10.16050/j.cnki.issn1674-6309.2020.02.005.
[17]
Miao J, Ye S, Lan J, et al. Nuclear HMGB1 promotes the phagocytic ability of macrophages[J]. Exp Cell Res, 2020, 393(1): 112037. DOI: 10.1016/j.yexcr.2020.112037.
[18]
Pezzanite L, Chow L, Piquini G, et al. Use of in vitro assays to identify antibiotics that are cytotoxic to normal equine chondrocytes and synovial cells[J]. Equine Vet J, 2021, 53(3): 579-589. DOI: 10.1111/evj.13314.
[19]
Azadpour M, Farajollahi MM, Varzi AM, et al. Extraction, Chemical Composition, Antioxidant Property, and In-vitro Anticancer Activity of Silymarin from Silybum marianum on Kb and A549 Cell Lines[J]. Curr Drug Discov Technol, 2021, 18(4): 511-517. DOI: 10.2174/1570163817666200827111127.
[20]
Wang JS, Ma D, Li Y, et al. Targeted delivery of CYP2E1 recombinant adenovirus to malignant melanoma by bone marrow-derived mesenchymal stem cells as vehicles[J]. Anticancer Drugs, 2014, 25(3): 303-314. DOI: 10.1097/CAD.0000000000000046.
[21]
Welling M, Kalyviotis K, Pantazis P. Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches[J]. Bio Protoc, 2020, 10(11): e3645. DOI: 10.21769/BioProtoc.3645.
[22]
Li J, Yang F, Jiang BY,et al. The synchronization of multiple signal amplifications for label-free and sensitive aptamer-based sensing of a protein biomarker[J]. Analyst, 2021, 145(24): 7858-7863. DOI: 10.1039/d0an01491e.
[23]
Li MH, Manathunga L, London E, et al. The Fluorescent Dye 1,6-Diphenyl-1,3,5-hexatriene Binds to Amyloid Fibrils Formed by Human Amylin and Provides a New Probe of Amylin Amyloid Kinetics[J]. Biochemistry, 2021, 60(25): 1964-1970. DOI: 10.1021/acs.biochem.1c00328.
[24]
Liu MY, Jiang LX. Advances in molecular imaging studies on in vivo tracing of exosomes[J]. Journal of Medical Research, 2021, 50(1): 145-148. DOI: 10.11969/j.issn.1673-548X.2021.01.031.
[25]
Zhang J, Qu C, Li TT, et al. Phagocytosis mediated by scavenger receptor class BI promotes macrophage transition during skeletal muscle regeneration[J]. J Biol Chem, 2019, 294(43): 15672-15685. DOI: 10.1074/jbc.RA119.008795.
[26]
Yan LL, Zaher HS. How do cells cope with RNA damage and its consequences?[J]. J Biol Chem, 2019, 294(41): 15158-15171. DOI: 10.1074/jbc.REV119.006513.
[27]
Chen XJ, Li X, Chen Q. Experimental Study of Ultrafine Superparamagnetic Iron Oxide-Enhanced MRI in an Atherosclerotic Plaque Model[J]. J Nanosci Nanotechnol, 2020, 20(12): 7444-7450. DOI: 10.1166/jnn.2020.18882.
[28]
Wang YS, Jiang LP, Zhang YW, et al. Fibronectin-Targeting and Cathepsin B-Activatable Theranostic Nanoprobe for MR/Fluorescence Imaging and Enhanced Photodynamic Therapy for Triple Negative Breast Cancer[J]. ACS Appl Mater Interfaces, 2020, 12(30): 33564-33574. DOI: 10.1021/acsami.0c10397.
[29]
Johnson JM, Mohamed ASR, Ding Y, et al. Ultra-small superparamagnetic iron oxide (USPIO) magnetic resonance imaging in benign mixed tumor of the parotid gland[J]. Clin Case Rep, 2021, 9(1): 123-127. DOI: 10.1002/ccr3.3477.

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