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Original Article
Experimental study of Gd-EOB-DTPA dynamic contrast-enhanced magnetic resonance imaging quantitative assessment of moderate liver fibrosis value in rats
GE Yansong  SHI mingqi  SUN linna  WANG Peiyuan 

Cite this article as: Ge YS, Shi MQ, Sun LN, et al. Experimental study of Gd-EOB-DTPA dynamic contrast-enhanced magnetic resonance imaging quantitative assessment of moderate liver fibrosis value in rats[J]. Chin J Magn Reson Imaging, 2022, 13(1): 91-97. DOI:10.12015/issn.1674-8034.2022.01.018.

[Abstract] Objective To investigate the value of ethoxybenzyl diethylene-triamine-pentaacetic-acid (Gd-EOB-DTPA)-dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) to quantify the value of moderate hepatic fibrosis (HF) in rats.Materials and Methods: A total of 40 male Wistar rats (10 in the control group and 30 in the experimental group) were intraperitoneally injected with CCl4 to construct HF models of different stages, and axial T1WI and DCE-MRI scans of liver were performed to obtain the following parameters: Volume transport constant (Ktrans), rate constant (Kep), percentage of extracellular space volume (Ve), and initial area under curve (iAUC). All rats were blooded intravenously to determine the content of hyaluronic acid (HA) and layer adhesive protein (LN) in the serum. The liver tissue of rats was taken for HF pathology stage. The difference between DCE-MRI parameters and serological indicators in normal group, normal group and mild HF group and moderate HF group was compared with independent sample t-test; the difference between DCE-MRI parameters and serological index in different HF stages was compared by using the single-factor variance analysis, the correlation between DCE-MRI parameter and serological indicators and HF stage were analyzed by using the Spearman correlation test;the value of moderate HF for diagnosis of each parameter is compared by using the ROC curve.Results A total of 26 rats were included in the study, of which 6 were pathologically staged for normal group, 7 were mild HF (F1) and 13 were moderate HF (F2—F3), all of DCE-MRI parameters (Ktrans, Ve, Kep and iAUC) decreased with HF (r=-0.665, P<0.001; r=-0.395, P=0.046; r=-0.565, P=0.003; r=-0.538, P=0.005), serum LN and HA content increased with HF (r=0.606, P=0.001; r=0.601, P=0.001); Ktrans, Ve and iAUC had statistical differences between the experimental group and the normal group, the normal group and the mild HF group and the moderate HF group, the normal group and the moderate HF group, the mild HF and the moderate HF group (P<0.05); the differences in serum LN and HA content between the control group and the moderate HF group were statistically significant (P<0.05); and the ROC curve shows that Ktrans and LN diagnose moderate HF with the highest efficiency.Conclusions Gd-EOB-DTPA DCE-MRI quantitative evaluation has good diagnostic value for moderate HF staged, of which Krans has the best diagnostic efficiency in DCE-MRI parameters.
[Keywords] hepatic fibrosis;gadolinium ethoxybenzyl diethylene-triamine-pentaacetic-acid;dynamic contrast-enhanced magnetic resonance imaging;capacity transfer constant

GE Yansong   SHI mingqi   SUN linna   WANG Peiyuan*  

Department of Medical Radiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, China

Wang PY, E-mail:

Conflicts of interest   None.

Received  2021-10-08
Accepted  2021-11-25
DOI: 10.12015/issn.1674-8034.2022.01.018
Cite this article as: Ge YS, Shi MQ, Sun LN, et al. Experimental study of Gd-EOB-DTPA dynamic contrast-enhanced magnetic resonance imaging quantitative assessment of moderate liver fibrosis value in rats[J]. Chin J Magn Reson Imaging, 2022, 13(1): 91-97.DOI:10.12015/issn.1674-8034.2022.01.018

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